Recombinant Human S100B
Catalog #: DCM19 Derived From: E.coli
Recombinant Human S100 Calcium Binding Protein B/S100B (N-6His) is produced by E.coli expression system and the target gene encoding Met1-Glu92 is expressed with a 6His tag at the N-terminus.
Names(Known as):Protein S100-B; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B; S100b; S100 beta; S100 calcium binding protein B
Mol Mass: 12.2kDa AP Mol Mass: 12kDa, reducing conditions
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Supplied as a 0.2 μM filtered solution of 20mM PBS,150mM NaCl,pH7.4.
The product is shipped on dry ice pack. Upon receipt, store it immediately at the temperature listed below.
Reconstituted protein solution should be stored at ≤ -20°C.
Affinity purification chromatography.
Immunogen, calibrator or standard.
S100-B, is an acidic protein with a molecular weight of 21 kDa belonging to the S100 family. S100-B contains two EF-hand-type calcium-binding motifs separated by a hinge region with a hydrophobic cleft. S100-B plays an important role in neurodevelopment, differentiation, and brain construction. S100-B has neuroprotective effects, but at high concentrations S100-B is neurotoxic. Extracellular concentration of S100-B increases following brain damage, which easily penetrates into cerebrospinal fluid in brain damage and then into the blood. S100-B is expressed and produced by astrocytes in vertebrate brains and in the CNS, and the astrocytes are the major cells producing S100-B protein in gray matter, as well as oligodendrocytes are the predominant S100-B in protein producing cells in white matter. The major advantage of using S100-B is that elevations in serum or CSF levels provide a sensitive measure for determining CNS injury at the molecular level before gross changes develop, enabling timely delivery of crucial medical intervention before irreversible damage occurs. In addition, S100-B, which is also present in human melanocytes, is a reliable marker for melanoma malignancy both in bioptic tissue and in serum.
FOR RESEARCH OR FURTHER MANUFACTURING USE ONLY