Recombinant Human Interleukin-17F is produced by our E.coli expression system and the target gene encoding Arg31-Gln163 is expressed.
蛋白编号
Q96PD4
产品别称
Interleukin-17F; IL-17F; Cytokine ML-1; IL17F
偶联
Unconjugated
分子量
15 KDa
表观分子量
15 KDa, reducing conditions
剂型描述
Lyophilized from a 0.2 μm filtered solution of 20mM PB, pH 7.4.
内毒素
< 1 EU/µg as determined by LAL test.
纯度-SDS-PAGE
Greater than 95% as determined by reducing SDS-PAGE.
生物活性-Cell Based Assay
Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 5-40 ng/ml.
生物活性-ELISA
Immobilized Human IL-17F(C030) at 2μg/ml (100 μl/well)can bind Biotinylated Human IL-17RA-His-Avi(CY78).The ED50 of Biotinylated Human IL-17RA-His-Avi(CY78) is 0.11 ug/ml.
生物活性-BLI
Loaded Biotinylated Human IL-17RA-His-Avi(CY78) on HIS1K Biosensor, can bind Human IL-17F(C030) with an affinity constant of 0.56 uM nM as determined in BLI assay.
Loaded Biotinylated Human IL-17RA-Fc(CY89) on Pro A Biosensor, can bind Human IL-17F(C030) with an affinity constant of 0.15uM as determined in BLI assay.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
运输条件
The product is shipped at ambient temperature.
Upon receipt, store it immediately at the temperature listed below.
保存条件
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
产品背景
Interleukin-17 is a potent pro-inflammatory cytokine produced by activated memory T cells. There are at least six members of the IL-17 family in humans and in mice. Today, IL-17 represents a family of structurally related cytokines that share a highly conserved C-terminal region but differ from one another in their N-terminal regions and in their distinct biological roles. The six known members of this family, IL-17A through IL-17F, are secreted as homodimers. IL-17F also regulates cartilage matrix turnover and inhibits angiogenesis.